The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus

نویسندگان

چکیده

Mixed viral infections in plants involving a potyvirus and other unrelated virus often result synergistic effects, with significant increases accumulation of the non-potyvirus partner, as case melon infected by Watermelon mosaic (WMV) crinivirus Cucurbit yellow stunting disorder (CYSDV). To further explore interaction between these two viruses, activity RNA silencing suppressors (RSSs) was addressed transiently co-expressed combinations heterologous products Nicotiana benthamiana leaves. While strong RSS WMV Helper Component Proteinase (HCPro) unaltered, including no evident additive effects observed when weaker CYSDV P25, an unexpected negative effect P1 found on P25. Analysis protein expression during assays showed that amount P25 not reduced P1. The detrimental action dose-dependent, subcellular localization fluorescently labeled variants coincidences both nucleus cytoplasm. Also, immunoprecipitation experiments tagged versions proteins. This novel interaction, previously described potyviruses criniviruses, might play role modulating complexities response to multiple susceptible plants.

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ژورنال

عنوان ژورنال: Frontiers in Microbiology

سال: 2021

ISSN: ['1664-302X']

DOI: https://doi.org/10.3389/fmicb.2021.645530